Journal: Human Molecular Genetics

Article Title: Restoration of muscle functionality by genetic suppression of glycogen synthesis in a murine model of Pompe disease

doi: 10.1093/hmg/ddp535

Figure Lengend Snippet: Expression of GLUT4 and GLUT1 glucose transporters. Protein levels were analyzed by western blot on plasma membranes (30 μg) isolated from tibialis anterior muscles of mice challenged with 0.2 IU/kg body weight insulin for 5 min. Loading was normalized using anti-M-cadherin antibody (A). Quantification of three independent experiments (B). Data are expressed as the mean ± SEM (n ≥ 4). *P < 0.05 compared with WT mice and #P < 0.05 compared with GAA/GYS1-KO mice. NS: non-significant difference compared with WT in the presence of insulin.

Article Snippet: Polyclonal antibodies were used to detect GLUT4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and GLUT1 (Chemicon/Millipore, Billerica, MA, USA).

Techniques: Expressing, Western Blot, Clinical Proteomics, Isolation, Muscles

Journal: Skeletal Muscle

Article Title: Muscle-specific loss of Bmal1 leads to disrupted tissue glucose metabolism and systemic glucose homeostasis

doi: 10.1186/s13395-016-0082-x

Figure Lengend Snippet: Both mRNA and protein expression of the glucose transporter type 4 (Glut4/GLUT4) were decreased in the iMSBmal1 -/- mice. a mRNA expression of Glut4 in the GTN muscle measured using RT PCR. (n = 6). b Protein content of GLUT4 in the GTN muscle was measured as integrated intensity of the fluorescent bands (n = 7)

Article Snippet: GLUT4 was detected using a primary monoclonal GLUT4 antibody (Cell Signaling, #2213) and an AlexaFluor680 goat anti-rabbit secondary antibody (Invitrogen, #A-21109).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction